何方：内科学（传染病学）博士，主任技师，硕士研究生导师，浙江省人民医院检验中心副主任。本科毕业于浙江大学应用生物科学专业，博士毕业于浙江大学传染病诊治国家重点实验室。主要从事临床分子生物学检验和细菌基因组学研究，近五年内主持国家自然科学基金两项，以通讯作者发表SCI论文23篇，入选浙江省2020年度“医坛新秀”培养对象，任中华医学会检验分会临床微生物学组委员，中华医学会医学病毒学分会青年委员，浙江省医学会医学病毒学分会常务委员，浙江省医学会检验分会青年委员会副主任委员。

 

·博士：2013 年9 月-2017 年2 月，浙江大学，内科学（传染病学）；
·硕士：2005 年9 月-2008 年6 月，浙江省医学科学院，病原生物学；
·学士：2000 年9 月-2004 年6 月，浙江大学，应用生物科学。
                   

 

1. 2020 年，浙江省医坛新秀培养对象；  

 

1. 中华医学会检验分会临床微生物学组委员；

2. 中华医学会医学病毒学分会青年委员；

3. 浙江省医学会医学病毒学分会常务委员；

4. 浙江省医学会检验分会青年委员会副主任委员。
   

       

 

1. 国家自然科学基金青年基金：碳青霉烯耐药肺炎克雷伯菌替加环素耐药机制研究，项目批准号：81702042，项目负责人。

2. 国家自然科学基金面上项目：tet(X)基因变异体介导革兰阴性杆菌替加环素高水平耐药的产生与传播机制研究，项目批准号：82172314，项目负责人。

3. 浙江省医药卫生科技项目：四环素相关耐药基因突变进化在碳青霉烯耐药肺炎克雷伯菌替加环素耐药中的作用研究，项目批准号：2019KY311，项目负责人。

4. 浙江省医药卫生科技项目：新型替加环素耐药基因tet(X4)在临床菌株中的传播机制和时空变化规律研究，项目批准号：2022KY022，项目负责人。

 

[1] He Fang^#, Fu Ying^#, Chen Qiong, Ruan Zhi, Hua Xiaoting, Zhou Hua, Yu Yunsong*. Tigecycline susceptibility and the role of efflux pumps in tigecycline resistance in KPC-producing Klebsiella pneumoniae. PLOS ONE. 2015;10(3): e0119064.  

[2] He Fang^#, Xu Juan^#, Wang Jianfeng, Chen Qiong, Hua Xiaoting, Fu Ying, Yu Yunsong*. Decreased susceptibility to tigecycline mediated by a mutation in mlaA in Escherichia coli strains. Antimicrobial agents and chemotherapy. 2016;60(12):7530-7531  

[3] Xu Juan^#, Gao Meng^#, Hong Yan, He Fang*. Draft genome sequence of a tigecycline-resistant KPC-2-producing Klebsiella pneumoniae ST15 clinical isolate from China. Journal of global antimicrobial resistance. 2017 Dec;11:156-158.  

[4] Sun Long^#, Xu Juan^#, He Fang*. Draft genome sequence of an NDM-5, CTX-M-15 and OXA-1 co-producing Escherichia coli ST167 clinical strain isolated from a urine sample. Journal of global antimicrobial resistance. 2018 Sep;14:284-286.  

[5] He Fang^#, Shi Qiucheng^#, Fu Ying, Xu Juan, Yu Yunsong, Du Xiaoxing*. Tigecycline resistance caused by rpsJ evolution in a 59-year-old male patient infected with KPC-producing Klebsiella pneumoniae during tigecycline treatment. Infection genetics and evolution. 2018;66:188-91.   

[6] Xu Juan, He Fang*. Genomic analysis of two bacterial strains co-isolated from a urinary tract infection: NDM-1-producing Enterobacter cloacae accompanied by extended-spectrum beta-lactamase-producing Escherichia coli. Journal of global antimicrobial resistance. 2019;17:198-200.  

[7] Xu Juan, He Fang*. Characterization of a NDM-7 carbapenemase-producing Escherichia coli ST410 clinical strain isolated from a urinary tract infection in China. Infection and drug resistance. 2019;12:1555-64.  

[8] Sun Long^#, Xu Juan^#, He Fang*. Genomic characterisation of a Proteus mirabilis clinical isolate from China carrying bla_NDM-5 on an IncX3 plasmid. Journal of global antimicrobial resistance. 2019;19:317-9. 

[9] Ruan Zhi, Wu Jianyong, Chen Hangfei, Draz MS, Xu Juan, He Fang*. Hybrid Genome Assembly and Annotation of a Pandrug-Resistant Klebsiella pneumoniae Strain Using Nanopore and Illumina Sequencing. Infection and drug resistance. 2020;13:199-206.  

[10] Xu Juan^#, Lin Wenping^#, Chen Yanmin, He Fang*. Characterization of an IMP-4-Producing Klebsiella pneumoniae ST1873 Strain Recovered from an Infant with a Bloodstream Infection in China. Infection and drug resistance. 2020;13:773-9.  

[11] Xu Juan^#, Zhao Zhao^#, Ge Yumei, He Fang*. Rapid Emergence of a Pandrug-Resistant Klebsiella pneumoniae ST11 Isolate in an Inpatient in a Teaching Hospital in China After Treatment with Multiple Broad-Spectrum Antibiotics. Infection and drug resistance. 2020;13:799-804.   

[12] Sun Long^#, Xu Juan^#, Wang Weizhong, He Fang*. Emergence of vanA-Type Vancomycin-Resistant Enterococcus faecium ST78 Strain with a rep2-Type Plasmid Carrying a Tn1546-Like Element Isolated from a Urinary Tract Infection in China. Infection and drug resistance. 2020;13:949-55. 

[13] Jiang Tao^#, Xu Juan^#, He Fang*. Genotypic and phylogenetic characterisation of a clinical Ralstonia pickettii strain carrying two novel OXA allelic variants, bla_OXA-898 and bla_OXA-899, isolated from a bloodstream infection in China. Journal of global antimicrobial resistance. 2020;21:46-8. 

[14] Xu Juan^#, Zhao Zhao^#, Ge Yumei, He Fang*. Unravelling the genome sequence of NDM-1 and KPC-2 co-producing Klebsiella pneumoniae ST11 isolated from a bloodstream infection. Journal of global antimicrobial resistance. 2020;20:339-41. 

[15] Xu Juan^#, Lin Wenping^#, Chen Yanming, He Fang*. Genomic and phylogenetic analysis of a community-acquired extended-spectrum β-lactamase-producing Escherichia coli ST429 strain recovered from a urinary tract infection. Journal of global antimicrobial resistance. 2020;22:656-658. 

[16] Zhu Zhongliang^#, Lu Jiawen^#, Chen Ying, He Fang*. Pasteurella canis infection caused by a dog bite leads to osteomyelitis and genomic analysis of the isolate. Journal of Clinical Laboratory Analysis. 2020;34(7):e23274. 

[17] Xu Juan, Zhu Zhongliang, Chen Yanmin, Wang Weizhong, He Fang*. The plasmid-borne tet(A) gene is an important factor causing tigecycline resistance in ST11 carbapenem-resistant Klebsiella pneumoniae under selective pressure. Frontiers in microbiology. 2021;12:644949. 

[18] Zhu Zhongliang^#, Xu Juan^#, He Fang*. Genomic and phylogenetic analysis of multidrug-resistant Achromobacter xylosoxidans ST273 strain MTYH1 co-carrying bla_OXA-114g and bla_CARB-2 recovered from a wound infection in China. Journal of global antimicrobial resistance. 2021;25:110-113. ISSN：2213-7165 

[19] Wang Weizhong, Xu Juan, Chen Ying, Zhu Zhongliang, He Fang*. An insight into the genome of a Listeria monocytogenes strain isolated from a bloodstream infection and phylogenetic analysis. Journal of clinical laboratory analysis. 2021;35(7):e23824. 

[20] Li Guoli#, Li Xinyang#, Wu Yuye, Xu Juan*, He Fang*. Genomic Insights into the Colistin Resistant mcr-Carrying Escherichia coli Strains in a Tertiary Hospital in China. antibiotics. 2022;11:1522.  

[21] Xu Juan#, Guo Hao#, Li Lirong, He Fang*. Molecular epidemiology and genomic insights into the transmission of carbapenem-resistant NDM-producing Escherichia coli. Computational and structural biotechnology journal. 2023;21:847-55.  

[22] Guo Hao#, Wu yuye#, Li Lirong, Wang jianfeng, Xu Juan*, He Fang*. Global emergence of carbapenem-resistant Klebsiella pneumoniae co-carrying multiple carbapenemases. Computational and structural biotechnology journal. 2023;21:3557-3563.

[23]Li L#, Zhang Y#, Guo H, Yang J, He F*. Genomic insights into a blaNDM-5-carrying Escherichia coli ST167 isolate recovered from faecal sample of a healthy individual in China. Journal of global antimicrobial resistance. 2024;36:240-243.

[24] Zhang Y#, Li Q#, Li L, Guo H, He F*. Characterization of a Novel Sequence Type (ST) 6758 Klebsiella Pneumoniae and the Role of IncX3 Plasmid in the Transmission of blaNDM. Infect Drug Resist. 2024;17:4935-4943.

[25] Guo H#, Li Q#, Zhang Y#, Lin W, Chen Y, He F*. Cefiderocol resistance landscape: Insights into the global spread of blaPER-1-carrying Gram-negative bacteria. The Journal of infection. 2025;91(4):106611.

 

目前在读硕士研究生3名，联合培养研究生1名。