卢觅佳，2004年毕业于浙江大学生物医学工程与仪器科学学院，获生物医学工程博士学位。2004至2014年于浙江省医学科学院工作，从事免疫毒理学；2014至2021年于美国俄亥俄州立大学兽医学院从事博士后工作，研究方向为病毒RNA的甲基化功能研究和病毒载体疫苗研发；2022年进入杭州医学院检验医学院，研究方向为RNA病毒的天然免疫逃逸机制研究。共参与发表SCI论文20多篇，其中以第一作者在Nature Microbiology 发表1篇、在PNAS发表3篇研究论文。目前主持一项国家自然科学基金面上项目。

 

2001.9.1—2004.6.10: 浙江大学生物医学工程与仪器科学学院生物医学工程系攻读博士学位， 获生物医学工程博士学位

1999.9.1—2001.7.30: 浙江大学动物科学学院兽医系攻读硕士学位，2001年提前攻博

1995.9.1—1999.6.2: 浙江大学动物科学学院学习，获兽医专业学士学位

 

2004～2014年：浙江省医学科学院，从事免疫毒理学；

2014～2021年：美国俄亥俄州立大学兽医学院，从事博士后工作，研究方向为病毒RNA的甲基化修饰在免疫逃逸中的功能和病毒载体疫苗研发；

2022年～ ：杭州医学院检验医学院

 

1.     获奖

1)    2009年浙江省医药卫生科技创新二等奖，病毒性载体基因药物（产品）临床前安全评价技术与方法研究，第2完成人

2)    2012年浙江省医药卫生科技创新一等奖，药物临床前安全性评价综合技术的建立与应用，第7完成人

2.     专利申请

已授权：

1)    Mark Peeples, Jianrong Li,Prosper N. Boyaka, Anzhong Li, Mijia Lu, Yuanmei Ma. RECOMBINANT VECTORS ENCODING ZIKA VIRUS PROTEIN SUBUNITS. 申请号：16/762, 836. 申请时间：2018.11.9, 公开号：20210171979, 公开时间：2021.6.10 （https://patents.justia.com/patent/20210171979）

2)    Jianrong Li, Mark E. Peeples, Chuan He, Stefan Niewiesk, Mijia Lu, Miaoge Xue, Zhijie Zhang, Boxuan Zhao.METHODS AND COMPOSITIONS FOR TREATING NEGATIVE-SENSE SINGLE-STRANDED RNA VIRUS. 申请号：16/762, 836. 申请时间：2019.10.18, 公开号：20220033783, 公开时间：2022.2.3（https://patents.justia.com/patent/20220033783）

申请中：

1)    Jianrong Li, Mijia Lu, Prosper Boyaka, Yuexiu Zhang. Live attenuated Mumps virus-based SARS-CoV-2 vaccines for infants and children. United States of America, 申请号：63/292, 875. 申请时间：2021.12.22

2)    Jianrong Li, Stefan Niewiesk, Mijia Lu, Anzhong Li, Xueya Liang. A Novel Live Attenuated Measles virus vaccine strains for SARS-CoV-2 vaccine; additional details and vaccine candidates.  PCT. 申请号：63/134, 111. 申请时间：2021.1.5

3)    Jianrong Li, Anzhong Li, Mijia Lu, Gibson Lanier. VSV-based SARS-CoV-2 vaccine candidates and IgY antibody as prophylactic and therapeutic agents against SARS-CoV-2 infection. United States of America. 申请号：63/194, 331. 申请时间：2021.5.28

4)    Jianrong Li, Niewiesk, S., Xue, M., Lu, M., Peeples, M.E., He, C., Zhang, Z., Zhao, B. Methods and compositions for treating human respiratory syncytial virus (RSV) and human metapneumovirus (hMPV). United States of America.申请号：17/309, 038. 申请时间：2021.4.16 

5)    Chuan He, Yuexiu Zhang, Mijia Lu, Jianrong Li. RNA m5C methylation as a novel target for antiviral therapy and immunotherapy. The Ohio State University and University of Chicago. Invention Disclosure.申请号：T2021-012. 申请时间：2020. 

3.     成果转化（License agreements/commercialization）:

Jianrong Li, Stefan Niewiesk, Mijia Lu, Anzhong Li, Xueya Liang. Novel Live Attenuated Measles Virus Vectored Vaccine. Platform for Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). Licensed to Biological E. Limited, India. Agreement reference: A2021-0232. 

 

浙江省医学会医学遗传学分会委员

浙江省细胞生物学学会免疫细胞生物学专业委员会委员

 

国家自然科学基金面上项目：N6甲基腺苷在人偏肺病毒逃逸树突细胞天然免疫中的作用机制研究

 

1.     Cai H, Zhang Y, Lu M, Liang X, Jennings R, Niewiesk S, Li J. Phosphorylation of human metapneumovirus M2-1 protein upregulates viral replication and pathogenesis. Journal of Virology, 2016, 90(16):7323-7338. 

2.     Wang Y, Liu R, Lu M, Yang Y, Zhou D, Hao X, Zhou D, Wang B, Li J, Huang YW, Zhao Z. Enhancement of safety and immunogenicity of the Chinese Hu191 measles virus vaccine by alteration of the S-adenosylmethionine (SAM) binding site in the large polymerase protein. Virology, 2018, 518: 210-220.

3.     McMichael TM, Zhang Y, Kenney AD, Zhang L, Zani A, Lu M, Chemudupati M, Li J, Yount JS. IFITM3 Restricts Human Metapneumovirus Infection. J. Infect. Dis. 2018, 218(10):1582-1591.

4.     Li A, Yu J, Lu M, Ma Y, Attia Z, Shan C, Xue M, Liang X, Craig K, Makadiya N, He JJ, Jennings R, Shi PY, Peeples ME, Liu SL, Boyaka PN, Li J*.  A Zika virus vaccine expressing premembrane-envelope-NS1 polyprotein. Nature Communications. 2018, 9: 3067. 

5.     Zhu Y, Ma Y, Lu M, Zhang Y, Li A, Liang X, Li J*. Efficient Production of Human Norovirus-Specific IgY in Egg Yolks by Vaccination of Hens with a Recombinant Vesicular Stomatitis Virus Expressing VP1 Protein. Viruses. 2019, 11(5): 444. 

6.     Xue M, Zhao BS, Zhang Z, Lu M, Harder O, Chen P, Lu Z, Li A, Ma Y, Xu Y, Liang X, Zhou J, Niewiesk S, Peeples ME, He C, Li J*. Viral N6-methyladenosine upregulates replication and pathogenesis of human respiratory syncytial virus. Nature Communications, 2019, 10(1): 4595. 

7.     Craig K, Dai X, Li A, Lu M, Xue M, Rosas L, Gao TZ, Niehaus A, Jennings R, Li J*. A Lactic Acid Bacteria (LAB)-Based Vaccine Candidate for Human Norovirus. Viruses. 2019 11(3): 213. 

8.     Xue M, Wang R, Harder O, Chen P, Lu M, Cai H, Li A, Liang X, Jennings R, La Perle K, Niewiesk S, Peeples ME, Li J*.  Stable attenuation of human respiratory syncytial virus for live vaccines by deletion and insertion of amino acids in the hinge region between the mRNA capping and methyltransferase domains of the large polymerase protein. Journal of Virology, 2020, 94(24): e01831-20. 

9.     Lu M, Zhang Z, Xue M, Zhao BS, Harder O, Li A, Liang X, Gao TZ, Xu Y, Zhou J, Feng Z, Niewiesk S, Peeples ME, He C, Li J*. N6-methyladenosine modification enables viral RNA to escape recognition by RNA sensor RIG-I. Nature Microbiology, 2020, 5(4): 584-598. 

10.  Li A, Xue M, Attia Z, Yu J, Lu M, Shan C, Liang X, Gao TZ, Shi PY, Peeples ME, Boyaka PN, Liu SL, Li J*. Vesicular stomatitis virus and DNA vaccines expressing Zika virus nonstructural protein 1 induce substantial but not sterilizing protection against Zika virus infection. Journal of Virology. 2020, 94(17): e00048-20. 

11.  Lu Y, Cai H, Lu M, Ma Y, Li A, Gao Y, Zhou J, Gu H, Li J*, Gu J*. Porcine epidemic diarrhea virus deficient in RNA cap guanine-N-7 methylation is attenuated and induces higher type I and III interferon responses.  Journal of Virology, 2020, 94 (16): e00447-20. 

12.  Lu M, Xue M, Wang HT, Kairis EL, Ahmad S, Wei J, Zhang Z, Liu Q, Zhang Y, Gao Y, Garcin D, Peeples ME, Sharma A, Hur S, He C, Li J*. Non-segmented negative-sense RNA viruses utilize N6-methyladenosine (m6A) as a common strategy to evade host innate immunity. Journal of Virology, 2021, 12; 95(9): e01939-20. 

13.  Lu M, Dravid P, Zhang Y, Trivedi S, Li A, Harder O, Kc M, Chaiwatpongsakorn S, Zani A, Kenney A, Zeng C, Cai C, Ye C, Liang X, Shimamura M, Liu SL, Mejias A, Ramilo O, Boyaka PN, Qiu J, Martinez-Sobrido L, Yount JS, Peeples ME, Kapoor A*, Niewiesk S*, Li J*. A safe and highly efficacious measles virus-based vaccine expressing SARS-CoV-2 stabilized prefusion spike. PNAS. 2021, 118 (12) e2026153118. 

14.  Terajima H, Lu M, Zhang L, Cui Q, Shi Y, Li J, He C*. N6-methyladenosine promotes induction of ADAR1-mediated A-to-I RNA editing to suppress aberrant antiviral innate immune responses. PLoS Biol. 2021, 19(7): e3001292. 

15.  Lu M, Zhang Y, Dravid P, Li A, Zeng C, Kc M, Trivedi S, Sharma H, Chaiwatpongsakorn S, Zani A, Kenney A, Cai C, Ye C, Liang X, Qiu J, Martinez-Sobrido L, Yount JS, Boyaka PN, Liu SL, Peeples ME, Kapoor A*, Li J*. A methyltransferase-defective VSV-based SARS-CoV-2 vaccine candidate provides complete protection against SARS-CoV-2 infection in hamsters. Journal of Virology, 2021, 95(20): e0059221. 

16.  Xue M, Zhang Y, Wang H, Kairis EL, Lu M, Ahmad S, Attia Z, Harder O, Zhang Z, Wei J, Chen P, Gao Y, Peeples ME, Sharma A, Boyaka P, He C, Hur S, Niewiesk S, Li J*. Viral RNA N6-methyladenosine modification modulates both innate and adaptive immune responses of human respiratory syncytial virus. PLoS Pathog. 2021, 17(12): e1010142. 

17.  Xiang J, Lu M, Shi M, Cheng X, Kwakwa KA, Davis JL, Su X, Bakewell SJ, Zhang Y, Fontana F, Xu Y, Veis DJ, DiPersio JF, Ratner L, Sanderson RD, Noseda A, Mollah S, Li J*, Weilbaecher KN*. Heparanase Blockade as a Novel Dual-Targeting Therapy for COVID-19. Journal of Virology. 2022 Mar 23:e0005722. 

18.  Zhang Y#, Lu M#, Mahesh KC, Kim E, Shamseldin MM, Ye C, Dravid P, Chamblee M, Park JG, Hall JM, Trivedi S, Chaiwatpongsakorn S, Kenny AD, Murthy SS, Sharma H, Liang X, Yount JS, Kapoor A, Martinez-Sobrido L, Dubey P, Boyaka PN, Peeples ME, Li J. A highly efficacious live attenuated mumps virus-based SARS-CoV-2 vaccine candidate expressing a six-proline stabilized prefusion spike. PNAS. 2022 Aug 16;119(33):e2201616119. （#：共同一作）

19.  Lu M, Chamblee M, Zhang Y, Ye C, Dravid P, Park JG, Mahesh KC, Trivedi S, Murthy S, Sharma H, Cassady C, Chaiwatpongsakorn S, Liang X, Yount JS, Boyaka PN, Peeples ME, Martinez-Sobrido L, Kapoor A, Li J. SARS-CoV-2 prefusion spike protein stabilized by six rather than two prolines is more potent for inducing antibodies that neutralize viral variants of concern. PNAS. 2022 Aug 30;119(35):e2110105119. 

20.  Zhang Y, Zhang LS, Dai Q, Chen P, Lu M, Kairis EL, Murugaiah V, Xu J, Shukla RK, Liang X, Zou Z, Cormet-Boyaka E, Qiu J, Peeples ME, Sharma A, He C, Li J. 5-methylcytosine (m5C) RNA modification controls the innate immune response to virus infection by regulating type I interferons. Proc Natl Acad Sci U S A. 2022 Oct 18;119(42):e2123338119. 

 

带教硕士研究生4名